Restriction Mapping of Retroviral Vector Episomal DNA
نویسندگان
چکیده
منابع مشابه
Restriction mapping of retroviral vector episomal DNA.
The infidelity of reverse transcription enzyme activity during the replication cycle of a retrovirus can cause retroviruses to rapidly mutate. For gene therapy, this raises biosafety issues, such as the deletion of a therapeutic gene from a vector and the development of a replication-competent retrovirus. In a single retroviral replication cycle, a therapeutic herpes simplex virus thymidine kin...
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Two algorithms are described that rapidly construct restriction maps of DNA molecules from single- and double-digest data. The implementation of these algorithms into the Pascal programs AMAPK and AMARD allows such mapping to be done on microcomputers.
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Restriction site maps of chloroplast DNA (cpDNA), maternally inherited in Clarkia, were obtained in representative species of all seven diploid sections, the allotetraploid sect. Clarkia, and the outgroup Epilobium brachycarpum. CpDNA restriction site variation was analyzed by Wagner and Dollo parsimony, bootstrapping, and genetic distance methods. Four shortest Wagner trees, one of which was a...
متن کاملInterspecies homology of nitrogenase genes ( recombinant DNA / restriction endonuclease mapping / DNA - DNA hybridization )
Cloned nitrogen fixation (nifl) genes from Klebsiella pneumoniae hybridize to DNA from 19 out of 19 widely divergent nitrogen-fixing bacterial strains but do not hybridize to DNA from 10 different non-nitrogen-fixing species. K pneumoniae nifDNA fragments that hybridize to DNA from other species contain part of the three structural genes that code for nitrogenase polypeptides. We have utilized ...
متن کاملInterspecies homology of nitrogenase genes ( recombinant DNA / restriction endonuclease mapping / DNA - DNA hybridization ) GARY B . RUVKUN
Cloned nitrogen fixation (nifl) genes from Klebsiella pneumoniae hybridize to DNA from 19 out of 19 widely divergent nitrogen-fixing bacterial strains but do not hybridize to DNA from 10 different non-nitrogen-fixing species. K pneumoniae nifDNA fragments that hybridize to DNA from other species contain part of the three structural genes that code for nitrogenase polypeptides. We have utilized ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00283cr01